Monitoring PCDM-specific biomarkers, galectin-3 and S100A9, to serve as a risk assessment of pancreatic cancer on new-onset diabetes patients for early detection of PC.

Summary Description： 1. Stress alters mitochondrial import of proteins. To monitor changes in mitochondrial import at single-mitochondrial resolution, split GFP epitopes are fused to the target proteins and mitochondrial membranes or expressed in the mitochondrial matrix. 2. General genotoxic agents damage both nuclear and mitochondrial DNA. By expressing mitochondrial DNA binding protein TFAM fused with KillerRed, specific mitochondrial DNA damages by light exposure are induced for investigating mitochondrial DNA damage signal. 3. Quantification of four dNTP pools is important for understanding drug action. The popularity of the analysis has been limited by the requirement of radio-isotope or sophisticated instrumentation. The method of click reaction we designed for measuring dNTPs overcomes the drawbacks of conventional methods. 4. The outbreak of drug-resistant Candida infection in hospital is of concern. We found the distinct biochemical property of Candida TMPK in high affinity to uridylate as the substrate, leading to the discovery of 5FUd (＜5 nM) in suppressing Candida growth.

The International Center for Wound Repair and Regeneration of National Cheng Kung University transferred and established Ducolege Biotechnology Co., Ltd., using Extre2Cold exclusive extraction technology to extract highly active marine collagen from Tilapia fish skin, which is type I collagen with a complete molecular structure and has a three-stranded helix structure. It is biologically active and perfectly presents the magical ability of fish skin to repair wounds. It can be widely used in biomedical materials and academic research. It is currently first promoted in the development of high-level medical beauty repair and maintenance products, which is helpful for repairing damaged skin.

In the current study, cell subsets, cell proportions, and the immune function of tumor-infiltrating leukocytes were used to indicate the features of tumor immune microenvironment in non-small cell lung cancers (NSCLCs) with different types of EGFR genetic alteration.

The core generated several types of transgenic mice and a stable humanized ACE2 mice using a combi-CRISPR genetic engineering technique. We hope these mouse models can help to dissect COVID-19 pathogenic mechanism and the interaction(s) between spike protein and ACE2 K353.

After a period of strict quarantine, these animals are continuously screened with the twin goals of finding specific pathogen-free shrimp seeds while maintaining genetic diversity. The best representatives of these two sub-goals are then selected for production at our commercial P. monodon shrimp seed expansion site in Hualien.

In the prior art of high-efficiency anode materials for lithium-ion batteries, we designed a high-entropy composition and manufactured it through a simple and mass production method. This technology inherits the cocktail effect of high-entropy alloys by combining highly active elements and metal elements. This material can be manufactured by a simple mass production method. It shows high energy density, high Coulomb efficiency and high cycle stability, and becomes a promising anode material for lithium-ion batteries.

The technology uses cathodic arc deposition combined with electromagnet field control and optical emission spectroscopy (OES) for the deposition of new functional high entropy alloy (HEA) nitride coatings. The HEA nitride coatings possessed such high hardness and excellent hydrophobicity with low surface energy which are beneficial for high speed dry cutting. Finally, the coatings were deposited on the micro end mills for cutting test of printed circuit board. The HEA ACZN coated tools can possess high wear resistance and outperform the uncoated tools at least 4 times of tool lives.

This technology is used to reveal the immune profiling of the tumor microenvironment based on multi-color immunohistochemistry staining. Each immune cell type will be probed with different fluorophores. Density, as well as the location of each immune cell type on the tissue slides, will be disclosed using inForm software.

This technology uses the ultrasonic non-destructive test technology to detect Li-ion battery packs which are commonly used in EVs. After the detection is completed, the ultrasonic signals are input into the AI diagnostic & evaluation model to obtain the status of each pouch cell in the battery pack.