SARS-CoV-2

SARS-CoV-2 IsolationWhole-genome Sequencing Analysis-The whole-genome sequencing was performed to examine 28 SARS-CoV-2 virus strains from patient tissue samples isolated by NTUH Department of Laboratory Medicine.2. Vh-chimera DNA as a New ctDNA Marker for Detecting the Existence of HBV-related HCC-About one-third of patients suffer tumor recurrence within the first year after surgical resection of HCCtheir overall survival is compromised. The study identified vh-chimera DNA as a novel ctDNA marker for HBV-related HCC detection.

A virus NT was performed in a biosafety level 3 laboratory to determine the titers. A SARS-CoV-2 enzyme-linked immunosorbent assay (ELISA) was designed to detect neutralizing antibodies in the serum, based on the binding affinity of SARS-CoV-2 viral protein 1 and viral protein 2 to antibodies. ELISA results viral protein 1 and 2, generated comparable neutralizing antibody titers. The results analyzed by spline regression and two-variable generalized additive model precisely reflected the real NT value. Our method can serve as a surrogate to quantify neutralizing antibody titer.

By using the machine learning algorithms, this study developed a risk stratification model for predicting the occurrence of in-hospital cardiac arrest (IHCA) events in patients admitted from the emergency department with COVID-19 and pneumonia. The results showed that the model's performance is better than by using the National Early Warning Score (NEWS).

e propose an AI dynamics drug repurposing platform applicate on COVID-19. This platform analyzed a large number of structures of SARS-CoV, MERS, and cross-species coronavirus 3CL protease-ligand complexes to construct uncovering six flexible active site conformations and pharmacophore clusters for SARS-CoV-2 3CL protease screening all FDA drugs and found four inhibitors within three months. Among them, JM206 had even demonstrated ten times better efficacy than Remdesivir in-vitro assay and also show the effect on the in-vivo hamster model to alleviate the symptoms caused by COVID-19.

The invention relates to a thermoplastic thin 2D mesh fabric composite structure, which particularly can be used as a medical protectorin connection with sporting activities with impact resistance, light weighthigh air permeability,has a reshapeable orthopedic support,fixed support bandages for limbs/joints. The thermoplastic thin 2D mesh fabric composite structure mainly includes a breathable mesh fabrica high molecular polymer coated on the breathable mesh fabric that can be reshapedreused when exposed to heat.

This study proposes a 5G C-V2I (Cellular Vehicle-to-Infrastructure) enabled intelligent trajectory predictionwarning system, which can be implemented in a framework including RSUs (Road Side Units) with radar detection ability5G edge computing servers. This study exploits artificial intelligence to predict instant trajectories of vehicles at crossroads. The resulting augmented-awareness navigation information is then broadcasted to road users through 5G C-V2I with low latency. In practical applications, road users can obtain real-time dynamics of surrounding vehicles so that their level of safety can be effectively enhanced.

The core generated several types of transgenic mice and a stable humanized ACE2 mice using a combi-CRISPR genetic engineering technique. We hope these mouse models can help to dissect COVID-19 pathogenic mechanism and the interaction(s) between spike protein and ACE2 K353.

PANCREASaver contains a “Pancreas Cancer (PC) automatic segmentation model” (image segmentation) and a “Pancreas Cancer (PC) analysis AI model” (image classification) that can read the DICOM format of postcontrast CT images directly for the automatic analysis process. After conducting prep-processing with image processing algorithms, C2FNAS is employed to illustrate the tumor position prior to the diagnosis conducted by CNN. The results can be provided to the physician for diagnostic reference so as to reduce early omissions and increase the detection rate of pancreatic cancer.

The technology optimizes the antigen protein expression system, so that the structural protein Erns of classical swine fever virus (CSFV) converts insoluble proteins into soluble proteins during the production process. The breakthrough of this technology allows the antigen protein to be applied to the development of an immune attachment screening platform, as a specialized differential diagnostic reagents can distinguish between virus-infected and vaccinated pigs, which can be applied to the monitoring system of virus transmission range and vaccination

The SPC process technology developed by our team has a patented cloth surface treatment and multiple layers of conductive circuit layers (Layout) without etching and transfer printing processes. It can not only be continuously automated production (piece to piece), but also Environmental protection, in line with the green trend of cleaner production.

Summary Description： 1. Stress alters mitochondrial import of proteins. To monitor changes in mitochondrial import at single-mitochondrial resolution, split GFP epitopes are fused to the target proteins and mitochondrial membranes or expressed in the mitochondrial matrix. 2. General genotoxic agents damage both nuclear and mitochondrial DNA. By expressing mitochondrial DNA binding protein TFAM fused with KillerRed, specific mitochondrial DNA damages by light exposure are induced for investigating mitochondrial DNA damage signal. 3. Quantification of four dNTP pools is important for understanding drug action. The popularity of the analysis has been limited by the requirement of radio-isotope or sophisticated instrumentation. The method of click reaction we designed for measuring dNTPs overcomes the drawbacks of conventional methods. 4. The outbreak of drug-resistant Candida infection in hospital is of concern. We found the distinct biochemical property of Candida TMPK in high affinity to uridylate as the substrate, leading to the discovery of 5FUd (＜5 nM) in suppressing Candida growth.

1.2 kV SiC Super Junction MOSFET: We have developed the first 1.2 kV SJ MOSFET in Taiwan in 2020. Its threshold voltage is 3.5 V, saturation current is about 12 A, and the specific on resistance is 8mΩ*cm2.

1. Identification of host factor, which is required for virus replication, by “EditCell Virology Platform”.2. The host factor will be as a target to develop antivirals.3. The antiviral effects of the candidates can be validated in vitroin vivo.4. Two FDA-approved drugs are repurposed for COVID-19 treatment.

This study proposes a 5G C-V2I (Cellular Vehicle-to-Infrastructure) enabled intelligent trajectory predictionwarning system, which can be implemented in a framework including RSUs (Road Side Units) with radar detection ability5G edge computing servers. This study exploits artificial intelligence to predict instant trajectories of vehicles at crossroads. The resulting augmented-awareness navigation information is then broadcasted to road users through 5G C-V2I with low latency. In practical applications, road users can obtain real-time dynamics of surrounding vehicles so that their level of safety can be effectively enhanced.

(1) Ｇel-type solid electrolytesAll solid-state electrolytes for Lithium Ion Battery (LIBs)(2) Fabrication of dye-sensitized solar cells by printing processits applications on Internet-of-Things (IoT) - MOST 2019 Award(3) Silicon-carbon coating anode materials for LIBs: SiLican startup company growthglobal business layout(4) Power enhanced Intermediate Temperature Solid State Fuel Cell (SOFC)(5) Worldwide highest conversion efficiency (19.4) perovskite solar cells(6) Industrial linkingProduct design : LevioPoleSmart motorcycle suitcase

Assisted by in-house AI deep compression toolchain (ezLabel, ezModel, ezQUANT, ezHybrid-M), the proposed technology supports automatic AI model design and optimization with the integrated performance of 120x model size reduction and 70x power reduction in 2D CNN model, and develops a world-first 1/2/4/8-bit CNN model realized by the developed high efficiency Hybrid fixed point CNN NPU (Hybrid-NPU), which has been verified in Xilinx ZCU102 FPGA and achieves the performance up to 2.5 TOPS(8-b)/ 20TOPS(1-b)＠28nm technology running at 550MHz and 4TOPS/W energy efficiency.

Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) recombinant antigen A1 inhibits the expression of CD163 on the cell surface by inducing the polarization of M1 macrophage subset, which can further effectively reduce virus invasion and viral infection. A1 can regulate the endogenous genes of alveolar macrophages to activate Th1-type immune response and enhance cellular immunity. Therefore, this technical invention will be beneficial to screen the new generation of PRRSV candidate structural vaccines.

1. The modular design of a electric breaker is proposed. A prototype of a solid-state circuit with an input voltage of 500 V and adjustable rated current including 50 A, 100 A, 150 A and 200 A. 2. Achieved zero-current cutoff within 110 µs using the characteristics of IGBTs to detect the current value. 3. A12 bits analog/digital converter is used to achieve a higher accuracy current detection effect when compared with the conventional analog circuit. It can also detect the fault current rising rate and correspond to different protection delay times according to the different fault current rising rate, so that the components in the solid-state breaker can withstand a lower fault current.

The "Viral Vaccine Cell Production Technology Platform" of this exhibition is based on 1. Host cellvirus matchcolonization technology 2. Cell-expressed virus receptor technology 3. Blocking antiviral mechanism of host cell 4. Immortalization technology of host cell 5. Serum-free suspension cell culture technology to produce high-immunity virus vaccine strains containing intact virus antigens.

Intelligent Portable Neurofeedback Training System: Highlights of our product: 1. High accurate EEG value (with rigorous hardware and software design and verification) 2. real-time feedback by computer screen or APP 3. can be used at home (easy to ware, easy to use)

AI農情調查之UAV群眾協作平台後台支援自動鑲嵌建模，更具備四項突破技術：(1)巨量影像格化技術；(2)平行運算技術；(3)任務規格標準化；(4)UAV任務媒合。致力於打造空中UBER協作服務，未來能應用於農作物分佈調查、大範圍災情調查、農業保險、農地違法使用調查與休耕補助調查等面向。

The project is to establish an AUV system capable of performing underwater exploration and ecological surveys in various shallow water areas over a long period. This system can automate the collection, analysis, and recording of coral reef ecosystems' imaging, acoustic and hydrological data in designated areas.

High entropy materials were proposeddeveloped by Professor Jien-Wei Yeh in 1995. He named, defined,elucidated “High-Entropy Alloys,” as well as established High-Entropy Materials Center to develop critical materials for smart machine, green technology, biomedical technology,national defense. Alloys with high strength, corrosion resistance,anti-bacterial capability are also developed for manufacturing knives, door handles, handrails, etc. to prevent infection during the post-epidemic era.

The International Center for Wound Repair and Regeneration of National Cheng Kung University transferred and established Ducolege Biotechnology Co., Ltd., using Extre2Cold exclusive extraction technology to extract highly active marine collagen from Tilapia fish skin, which is type I collagen with a complete molecular structure and has a three-stranded helix structure. It is biologically active and perfectly presents the magical ability of fish skin to repair wounds. It can be widely used in biomedical materials and academic research. It is currently first promoted in the development of high-level medical beauty repair and maintenance products, which is helpful for repairing damaged skin.

Electroplated nanotwinned Cu possesses excellent electrical & mechanical properties. It can be applied in three major joints:1. Low thermal budget/ low resistance Cu bonding for high performance computing chip.We are able to achieve low temperature bondinginstant bonding. Low temperature bonding is performed at 150°C for 1 h to achieve low contact resistance copper bonding. Instant bonding is performed at 300°C for 5 seconds under a pressure of 90MPa achieve low contact resistance.2. High strength/ High ductility copper lines in 3D-IC packaging We are able to fabricate high strength foils with tensile strength of 800MPa. After annealing at 150°C for 3 hours, the foil retains a tensile strength of 750MPa. 3. Cu foils for lithium ion battery

PCA-BM includes two models: “Automatic BMs Segmentation AI Model” and " Distant Brain Failure Prediction Model". The former one uses C2FNAS (coarse to fine network architecture search) to detect the location, size, and the number of brain metastases. The latter uses radiomics to extract numerous radiographic features and employs machine learning methods such as XGBoost to establish a prognostic model of brain metastases. PCA-BM provides more precision treatment decisions for patients and improves personalized and accurate overall stereotactic radiosurgery planning.

NTU Medical Genie Precision Health Platform: The product is mainly composed of wearable devices, IoT environmental sensors, deep learning, personal health app and case management platform. It can collect and monitor user's lifestyle and environment automatically, and predict the possibility of emergency to assist medical staff in making decisions. In addition, we opened source the project to solve most clinical studies that require lots of time to build data collection tools and processes.

A famous commercial ad stated: “If the liver is unwell, life becomes black and white.” Those who have seen this are reminded to take good care of the liver, however liver disease is still quite prevalent in Taiwan. 13,000 deaths each year are due to some form of liver disease. Liver cancer is the 2nd leading cause of death in Taiwan and chronic liver disease & cirrhosis are the 9th leading cause of death.

This technology uses the ultrasonic non-destructive test technology to detect Li-ion battery packs which are commonly used in EVs. After the detection is completed, the ultrasonic signals are input into the AI diagnostic & evaluation model to obtain the status of each pouch cell in the battery pack.

Combined with millimeter wave radar detection of chest undulation breathing mode and heart rate, continuous blood oxygen detection, active disease record of chat robot, and mobile phone analysis of 30 second sitting and standing alternate activity frequency mode, a set of personalized respiratory capacity benchmark is established through AI modeling, which can be applied to zero-contact respiratory physiological monitoring and useful for infectious disease ward, epidemic prevention hotels, centralized quarantine centers.